![]() ![]() pylori strains are divided into seven types based on their geographical associations: HpEurope, hpEastAsia, hpAfrica1, hpAfrica2, hpAsia2, hpNEAfrica, and hpSahul. However, MLST only reflects the variability of several housekeeping genes and the method is costly. In this technique, the data saved in the central database are accessible via the Internet and provides a potential source for global epidemiological study. MLST is a rapid and reliable method that based on allelic variation using the polymorphism of seven housekeeping genes has the advantages of high repeatability and high resolution and can provide more detailed information on human migration than human genetic analysis to a certain extent. pylori genotyping methods include multi-locus sequence typing (MLST), random amplification of polymorphic DNA, pulsed-field gel electrophoresis, whole-genome sequencing, amplified fragment length polymorphism, and others. pylori population are clinically important. Despite the high prevalence of infection in Africa and South Asia, the incidence of gastric cancer is much lower in these areas than in other countries, and the malignancies of this infection decrease from North to Southeast Asia. , Besides, differences in gastric adenocarcinoma, chronic atrophic gastritis, and duodenal ulcers are partly associated with the geographical origins of strains. pylori strains of East Asia (Singapore and Korea) are distinct from European strains. Genetic studies indicate that geographical and climatic contexts affect a high diversity of H. Therefore, it can be used as a reliable biological marker for the simultaneous evolution of host–pathogen and ancient human migration based on the diversity of sequences at the selected gene site. Given that the microorganism has been probably in the human stomach for millions of years, it is considered to have evolved with its host. pylori is a panmictic species in which no diagnosable strong clonal structure exists. This feature allows the exchange of genetic matter between different strains living together in a stomach. pylori is naturally competent and can integrate a foreign DNA with homologous recombination into its genome. ![]() Extensive allelic diversity, genetic variability, mutations, and high recombination are prominent features of this microorganism. Infections caused by this bacterium are usually acquired in childhood and can remain asymptomatic for several years. pylori) is a Gram-negative spiral bacillus isolated from patients with chronic gastritis (CG) by Marshall and Warren in 1984. Genetic diversity of Helicobacter pylori isolates from patients with gastric diseases in Isfahan. How to cite this URL: Vazirzadeh J, Karbasizadeh V, Falahi J, Moghim S, Narimani T, Rafiei R. How to cite this article: Vazirzadeh J, Karbasizadeh V, Falahi J, Moghim S, Narimani T, Rafiei R. Keywords: Helicobacter pylori, multilocus sequence typing, prevalence Sequence analysis and determination of the origin of the phylogeny of strains can be effective in clinical management and monitoring of risk factors for chronic and recurrence of infection. pylori infection in this region, early and accurate identification of patients seems necessary. Conclusion: Given the high prevalence of H. The results of the global analysis showed that all the isolates, with a wide diversity, have a genetic affinity with members of the European population, such as Italy and Russia, and are in the hpEurope haplotype. In the results of the analysis of MLST, a total of 14 new STs were recorded. The phylogenetic tree was plotted using CLC v8 and iTol software. MLST of seven housekeeping genes was performed for 20 H. pylori infection was determined by FISH in paraffin-embedded biopsy specimens. Shariati Hospital of Isfahan, Iran, from April to July 2018. Materials and Methods: In this descriptive cross-sectional study, 165 gastric biopsy specimens were obtained from patients with dyspepsia referred to Dr. pylori isolates from patients with dyspepsia by the multi-locus sequence typing (MLST) and update data on the prevalence of H. This study aimed to determine the genetic diversity of H. This pathogen is highly diverse and can be used as genetic markers for predict the history of human migrations. pylori), a spiral-shaped bacterium colonizing the human stomach, is generally acquired in childhood. ![]()
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